One-stage and chromogenic FVIII:C assay discrepancy in mild haemophilia A and the relationship with the mutation and bleeding phenotype.

Autores de IIS La Fe

• Ana Rosa Cid Haro

  Autor

• Vicente Cortina Giner

  Autor

• Pilar Casaña Gargallo

  Autor

• Saturnino Haya Guaita

  Autor

• Andres Moret Peiro

  Autor

• Noelia Cabrera Garcia-Ochoa

  Autor

• Jose Antonio Aznar Lucea

  Autor

Participantes ajenos a IIS La Fe

• Calabuig M

Abstract

The discrepancy of the levels of factor VIII activity (FVIII:C) by different assays in some mild and moderate haemophilic A patients has been long known. Specific mutations affecting FVIII:C discrepancies have been described. No consensus exit as to which method most accurately represents the FVIII cofactor function in vivo and which has a better correlation with the haemorrhagic clinical expression. We studied 163 mild A haemophiliacs, and detected discrepancies in 20% of the patients, most of whom presented higher levels of FVIII:C with the one-stage assay. In nine families, the FVIII mutation was found, while three showed mutations not previously described (Leu1978Phe and Ser1791Pro associated with higher levels of FVIII:C by one-stage method; Arg1639His in a patient with low level of FVIII:C by the one-stage, but normal, chromogenic assay). Assessing the level of FVIII:C by different methods could help to learn the possible haemorrhagic expressions of patients.