Increased lung neutrophil apoptosis and inflammation resolution in nonresponding pneumonia.

Autores de IIS La Fe

• Inés Moret Tatay

  Autor

• Maria Jose Lorenzo Dus

  Autor

• Benjamin Sarria Chust

  Autor

• Enrique Cases Viedma

  Autor

• Miguel José Perpiña Tordera

  Autor

• José Miguel Molina Moreno

  Autor

• Rosario Menéndez Villanueva

  Autor

Participantes ajenos a IIS La Fe

• Morcillo E

Grupos

• Enfermedad Inflamatoria Intestinal

  

  Leader

  Pilar Nos Mateu

• Infecciones Respiratorias

  Investigador Principal

  Raúl Méndez Ocaña

Abstract

Neutrophil activation state and its relationship with an inflammatory environment in community-acquired pneumonia (CAP) remain insufficiently elucidated. We aimed to evaluate the neutrophil apoptosis and cytokine pattern in CAP patients after 72 h of treatment, and their impact on infection resolution. Apoptosis of blood and bronchoalveolar lavage (BAL) neutrophils was measured in nonresponding CAP (NCAP), in responding CAP (blood only) and in patients without infection (control). Pro-inflammatory (interleukin (IL)-6, IL-8) and anti-inflammatory (IL-10) cytokines were measured. Main outcomes were clinical stability and days of hospitalisation. Basal neutrophil apoptosis was higher in the BAL and blood of NCAP, whereas spontaneous apoptosis (after 24 h culture) was lower. Cytokines in NCAP were higher than in responding CAP and control: IL-6 was increased in BAL and blood, IL-8 in BAL and IL-10 in blood. An increased basal apoptosis (=20%) in BAL of NCAP was associated with lower systemic IL-10 (p<0.01), earlier clinical stability (p=0.05) and shorter hospital stay (p=0.02). A significant correlation was found for systemic IL-6 and IL-10 with days to reach stability and length of stay. After 72 h of treatment, an increased basal alveolar neutrophil apoptosis might contribute to downregulation of inflammation and to faster clinical stability.