A kinetic study on the regeneration of Candida albicans protoplasts in the presence of cell wall synthesis inhibitors.

Autores de IIS La Fe

• Jaime Font De Mora Sainz

  Autor

• Elena Herrero Gil

  Autor

Participantes ajenos a IIS La Fe

• Sentandreu R

Abstract

Aculeacin A and papulacandin B block cell wall regeneration in Candida albicans protoplasts at an intermediate step in which the protoplasts have not yet synthesized the rigid structure of the cell wall and are therefore still osmotically sensitive. In the presence of the antibiotics, total synthesis of glucan is not significantly lowered with respect to control cells, although most of it appears either in the culture medium or in the regenerating wall as alkali-soluble glucan. Thus, it is proposed that echinocandins (such as aculeacin A) and papulacandins may not inhibit glucan synthesis per se but instead inhibit its incorporation into the supramolecular organization of the cell wall.