The earliest thymic T cell progenitors sustain B cell and myeloid lineage potential.

Autores de IIS La Fe

• Alejandra Sanjuan Pla

  Autor

Participantes ajenos a IIS La Fe

• Luc S
• Luis TC
• Boukarabila H
• Macaulay IC
• Buza-Vidas N
• Bouriez-Jones T
• Lutteropp M
• Woll PS
• Loughran SJ
• Mead AJ
• Hultquist A
• Brown J
• Mizukami T
• Matsuoka S
• Ferry H
• Anderson K
• Duarte S
• Atkinson D
• Soneji S
• Domanski A
• Farley A
• Carella C
• Patient R
• de Bruijn M
• Enver T
• Nerlov C
• Blackburn C
• Godin I
• Jacobsen SE

Abstract

The stepwise commitment from hematopoietic stem cells in the bone marrow to T lymphocyte-restricted progenitors in the thymus represents a paradigm for understanding the requirement for distinct extrinsic cues during different stages of lineage restriction from multipotent to lineage-restricted progenitors. However, the commitment stage at which progenitors migrate from the bone marrow to the thymus remains unclear. Here we provide functional and molecular evidence at the single-cell level that the earliest progenitors in the neonatal thymus had combined granulocyte-monocyte, T lymphocyte and B lymphocyte lineage potential but not megakaryocyte-erythroid lineage potential. These potentials were identical to those of candidate thymus-seeding progenitors in the bone marrow, which were closely related at the molecular level. Our findings establish the distinct lineage-restriction stage at which the T cell lineage-commitment process transits from the bone marrow to the remote thymus.