Mass spectrometric detection of biomarkers for early assessment of intraamniotic fluid infection.

Fecha de publicación: 01/12/2015

Autores de IIS La Fe

Consuelo Cháfer Pericás

Autor
Ángel Sánchez Illana

Autor
Justo Javier Escobar Cubiella

Autor
María Cernada Badía

Autor
Elena Cubells Garcia

Autor
Antonio Guillermo Núñez Ramiro

Autor
Máximo Vento Torres

Autor
Julia Kuligowski

Autor

Participantes ajenos a IIS La Fe

Stefanovic V
Andersson S

Grupos

Perinatología

Leader

Máximo Vento Torres

Abstract

This data article contains information on glutathione sulfonamide (GSA) structural confirmation and purity after synthesis, as well as mass spectrometry acquisition parameters for the determination of GSA and other biomarkers for the early assessment of intraamniotic fluid infection in amniotic fluid samples (Chafer-Pericas et al., 2015) [1]. GSA standards were synthesized and structural confirmation was carried out employing time-of-flight mass spectrometry (TOF-MS); purity was assessed by high performance liquid chromatography (HPLC) with UV detection. For optimization of the acquisition parameters of GSA and other biomarkers, individual analytical standard solution at a concentration of 1 micromol L(-) (1) was injected into an Acquity - Xevo TQ liquid-chromatography coupled to tandem mass spectrometry (LC-MS/MS) system from Waters (Milford, MA, USA) operating in the positive electrospray (ESI(+)) mode. Mass spectrometric detection of 3-nitro-tyrosine (3NO2-Tyr), 3-chloro-tyrosine (3Cl-Tyr), 8-hydroxy-2'-deoxyguanosine (8OHdG), GSA and oxidized glutathione (GSSG) was carried out by multiple reaction monitoring (MRM). Linear response curves were calculated for each analyte normalizing the signal with peak areas of internal standards.

Datos de la publicación

ISSN/ISSNe:: 2352-3409,
Tipo:: Article
Páginas:: 1026-1030
DOI:: 10.1016/j.dib.2015.11.020
Factor de Impacto:: 0,187 SCImago ℠
Cuartil:: Q3 SCImago ℠

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Métricas

Filiaciones

Cháfer-Pericás C:: Instituto de Investigación. Neonatal Research Unit, Health Research Institute La Fe, Valencia, Avenida Fernando Abril Martorell 106, 46026 Valencia, Spain
Stefanovic V:: Department of Obstetrics and Gynecology, Fetomaternal Medical Center, Helsinki University Central Hospital and University of Helsinki, Haartmaninkatu 2, 00029 Helsinki, Finland
Sánchez-Illana Á:: Instituto de Investigación. Neonatal Research Unit, Health Research Institute La Fe, Valencia, Avenida Fernando Abril Martorell 106, 46026 Valencia, Spain
Escobar J:: Instituto de Investigación. Neonatal Research Unit, Health Research Institute La Fe, Valencia, Avenida Fernando Abril Martorell 106, 46026 Valencia, Spain
Cernada M:: Instituto de Investigación. Neonatal Research Unit, Health Research Institute La Fe, Valencia, Avenida Fernando Abril Martorell 106, 46026 Valencia, Spain
Cubells E:: Instituto de Investigación. Neonatal Research Unit, Health Research Institute La Fe, Valencia, Avenida Fernando Abril Martorell 106, 46026 Valencia, Spain
Núñez-Ramiro A:: Instituto de Investigación. Neonatal Research Unit, Health Research Institute La Fe, Valencia, Avenida Fernando Abril Martorell 106, 46026 Valencia, Spain
Andersson S:: Children?s Hospital, University of Helsinki and Helsinki University Hospital, Stenbäckinkatu 11, PO Box 281, 00029 Helsinki, Finland
Vento M:: Division of Neonatology, University & Polytechnic Hospital La Fe, Avenida Fernando Abril Martorell 106, 46026 Valencia, Spain
Kuligowski J:: Instituto de Investigación. Neonatal Research Unit, Health Research Institute La Fe, Valencia, Avenida Fernando Abril Martorell 106, 46026 Valencia, Spain

Keywords

Biomarker detection; Glutathione sulfonamide (GSA); High performance liquid chromatography (HPLC); Intraamniotic fluid infection; Liquid-chromatography coupled to tandem mass spectrometry (LC-MS/MS); Time-of-flight mass spectrometry (TOF-MS)