Mass spectrometric detection of biomarkers for early assessment of intraamniotic fluid infection.

Autores de IIS La Fe

• 

  María Consuelo Cháfer Pericás

  Autor

• Ángel Sánchez Illana

  Autor

• Justo Javier Escobar Cubiella

  Autor

• María Cernada Badía

  Autor

• Elena Cubells Garcia

  Autor

• Antonio Guillermo Núñez Ramiro

  Autor

• Máximo Vento Torres

  Autor

• Julia Kuligowski

  Autor

Participantes ajenos a IIS La Fe

• Stefanovic V
• Andersson S

Grupos

• Perinatología

Abstract

This data article contains information on glutathione sulfonamide (GSA) structural confirmation and purity after synthesis, as well as mass spectrometry acquisition parameters for the determination of GSA and other biomarkers for the early assessment of intraamniotic fluid infection in amniotic fluid samples (Chafer-Pericas et al., 2015) [1]. GSA standards were synthesized and structural confirmation was carried out employing time-of-flight mass spectrometry (TOF-MS); purity was assessed by high performance liquid chromatography (HPLC) with UV detection. For optimization of the acquisition parameters of GSA and other biomarkers, individual analytical standard solution at a concentration of 1 micromol L(-) (1) was injected into an Acquity - Xevo TQ liquid-chromatography coupled to tandem mass spectrometry (LC-MS/MS) system from Waters (Milford, MA, USA) operating in the positive electrospray (ESI(+)) mode. Mass spectrometric detection of 3-nitro-tyrosine (3NO2-Tyr), 3-chloro-tyrosine (3Cl-Tyr), 8-hydroxy-2'-deoxyguanosine (8OHdG), GSA and oxidized glutathione (GSSG) was carried out by multiple reaction monitoring (MRM). Linear response curves were calculated for each analyte normalizing the signal with peak areas of internal standards.

Keywords

• Biomarker detection; Glutathione sulfonamide (GSA); High performance liquid chromatography (HPLC); Intraamniotic fluid infection; Liquid-chromatography coupled to tandem mass spectrometry (LC-MS/MS); Time-of-flight mass spectrometry (TOF-MS)