Changes of the plasma metabolome of newly born piglets subjected to postnatal hypoxia and resuscitation with air

Fecha de publicación: 01/08/2016

Autores de IIS La Fe

Julia Kuligowski

Autor
Justo Javier Escobar Cubiella

Autor
Guillermo Quintas Soriano

Autor
Isabel Lliso Escudero

Autor
Ángel Sánchez Illana

Autor
Máximo Vento Torres

Autor

Participantes ajenos a IIS La Fe

Solberg R
Pankratov L
Saugstad OD

Grupos

Perinatología

Leader

Máximo Vento Torres

Abstract

BACKGROUND: Perinatal hypoxic-ischemic brain damage is a major cause of mortality and morbidity in the neonatal period. Currently, limited ranges of biochemical tests assessing the intensity and duration of hypoxia are ready for clinical use. However, the need to initiate hypothermia therapy early after the clinical suspicion of hypoxic-ischemic encephalopathy requires the availability of early and reliable hypoxia markers. We have sought these biomarkers in an experimental model of hypoxia reoxygenation. METHODS: Hypoxia and hypotension were induced in newborn piglets following a standardized model and reoxygenation was carried out using room air (RA). An untargeted liquid chromatography-time of flight mass spectrometry (LC-TOFMS) approach was used to assess changes in the metabolomic profile of plasma samples after intense hypoxia and upon reoxygenation. RESULTS: At the end of hypoxia, the plasma metabolome showed an increased plasma concentration of analytes reflecting a metabolic adaptation to prolonged anaerobiosis. However, after resuscitation, metabolite levels returned to the starting values. CONCLUSION: Severe hypoxia induces early, significant, and transient changes of specific metabolites in the plasma metabolome, which represent a snapshot of the biochemical adaptation of mammals to intense hypoxia. These metabolites could have applicability in predicting the severity of hypoxia in the clinical setting.

Datos de la publicación

ISSN/ISSNe:: 0031-3998, 1530-0447
Tipo:: Article
Páginas:: 284-292
DOI:: 10.1038/pr.2016.66
Factor de Impacto:: 1,439 SCImago ℠
Cuartil:: Q1 SCImago ℠

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Métricas

Filiaciones

Solberg R:: Department of Pediatric Research, Institute for Surgical Research, University of Oslo, Oslo University Hospital - Rikshospitalet, Oslo, Norway
Kuligowski J:: Instituto de Investigación. Neonatal Research Group, Health Research Institute Hospital La Fe, Valencia, Spain
Pankratov L:: Department of Pediatric Research, Institute for Surgical Research, University of Oslo, Oslo University Hospital - Rikshospitalet, Oslo, Norway
Escobar J:: Instituto de Investigación. Neonatal Research Group, Health Research Institute Hospital La Fe, Valencia, Spain
Quintás G:: Leitat Technological Center, Safety and Sustainability Division, Valencia, Spain
Lliso I:: Instituto de Investigación. Neonatal Research Group, Health Research Institute Hospital La Fe, Valencia, Spain
Sánchez-Illana Á:: Instituto de Investigación. Neonatal Research Group, Health Research Institute Hospital La Fe, Valencia, Spain
Saugstad OD:: Department of Pediatric Research, Institute for Surgical Research, University of Oslo, Oslo University Hospital - Rikshospitalet, Oslo, Norway
Vento M:: Instituto de Investigación. Neonatal Research Group, Health Research Institute Hospital La Fe, Valencia, Spain

Division of Neonatology, University & Polytechnic Hospital La Fe, Valencia, Spain