Changes of the plasma metabolome of newly born piglets subjected to postnatal hypoxia and resuscitation with air

Autores de IIS La Fe

• Julia Kuligowski

  Autor

• Justo Javier Escobar Cubiella

  Autor

• Guillermo Quintas Soriano

  Autor

• Isabel Lliso Escudero

  Autor

• Ángel Sánchez Illana

  Autor

• Máximo Vento Torres

  Autor

Participantes ajenos a IIS La Fe

• Solberg R
• Pankratov L
• Saugstad OD

Grupos

• Perinatología

Abstract

BACKGROUND: Perinatal hypoxic-ischemic brain damage is a major cause of mortality and morbidity in the neonatal period. Currently, limited ranges of biochemical tests assessing the intensity and duration of hypoxia are ready for clinical use. However, the need to initiate hypothermia therapy early after the clinical suspicion of hypoxic-ischemic encephalopathy requires the availability of early and reliable hypoxia markers. We have sought these biomarkers in an experimental model of hypoxia reoxygenation. METHODS: Hypoxia and hypotension were induced in newborn piglets following a standardized model and reoxygenation was carried out using room air (RA). An untargeted liquid chromatography-time of flight mass spectrometry (LC-TOFMS) approach was used to assess changes in the metabolomic profile of plasma samples after intense hypoxia and upon reoxygenation. RESULTS: At the end of hypoxia, the plasma metabolome showed an increased plasma concentration of analytes reflecting a metabolic adaptation to prolonged anaerobiosis. However, after resuscitation, metabolite levels returned to the starting values. CONCLUSION: Severe hypoxia induces early, significant, and transient changes of specific metabolites in the plasma metabolome, which represent a snapshot of the biochemical adaptation of mammals to intense hypoxia. These metabolites could have applicability in predicting the severity of hypoxia in the clinical setting.