Development of a reliable analytical method to determine lipid peroxidation biomarkers in newborn plasma samples

Fecha de publicación: 01/06/2016

Autores de IIS La Fe

Consuelo Cháfer Pericás

Autor
María Isabel Torres Cuevas

Autor
Ángel Sánchez Illana

Autor
Justo Javier Escobar Cubiella

Autor
Julia Kuligowski

Autor
Máximo Vento Torres

Autor

Participantes ajenos a IIS La Fe

Solberg R
Garberg HT
Huun MU
Saugstad OD

Grupos

Perinatología

Leader

Máximo Vento Torres

Abstract

This paper describes a reliable analytical method based on ultra-performance liquid chromatography coupled to tandem mass spectrometry to determine F2-isoprostanes and other total byproducts (isoprostanes, isofurans, neuroprostanes and neurofurans) as lipid peroxidation biomarkers in newborn plasma samples. The proposed procedure is characterized by a simple sample treatment employing a reduced sample volume (100 mu L). Also, it shows a high throughput and high selectivity to determine simultaneously different isoprostane isomers in a large number of samples. The reliability of the described method was demonstrated by analysis of spiked plasma samples, obtaining recoveries between 70% and 130% for most of the analytes. Taking into account the implementation of further clinical studies, it was demonstrated the proper sensitivity of the method by means of the analysis of few human newborn plasma samples. In addition to this, newborn piglet plasma samples (n=80) were analyzed observing that the developed method was suitable to determine the analyte levels present in this kind of samples. Therefore, this analytical method could be applied in further clinical research about establishment of reliable lipid peroxidation biomarkers employing this experimental model. (C) 2016 Elsevier B.V. All rights reserved.

Datos de la publicación

ISSN/ISSNe:: 0039-9140, 1873-3573
Tipo:: Article
Páginas:: 152-157
DOI:: 10.1016/j.talanta.2016.03.010
Factor de Impacto:: 1,168 SCImago ℠

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Filiaciones

Cháfer-Pericás C:: Instituto de Investigación. Neonatal Research Group, Health Research Institute La Fe, Valencia, Spain.
Torres-Cuevas I:: Instituto de Investigación. Neonatal Research Group, Health Research Institute La Fe, Valencia, Spain
Sanchez-Illana A:: Instituto de Investigación. Neonatal Research Group, Health Research Institute La Fe, Valencia, Spain
Escobar J:: Instituto de Investigación. Neonatal Research Group, Health Research Institute La Fe, Valencia, Spain
Kuligowski J:: Instituto de Investigación. Neonatal Research Group, Health Research Institute La Fe, Valencia, Spain
Solberg R:: Department of Pediatric Research, University of Oslo, Oslo University Hospital, Rikshospitalet, Norway
Garberg HT:: Department of Pediatric Research, University of Oslo, Oslo University Hospital, Rikshospitalet, Norway
Huun MU:: Department of Pediatric Research, University of Oslo, Oslo University Hospital, Rikshospitalet, Norway
Saugstad OD:: Department of Pediatric Research, University of Oslo, Oslo University Hospital, Rikshospitalet, Norway
Vento M:: Division of Neonatology, University & Polytechnic Hospital La Fe, Valencia, Spain