A Fluorogenic Biosensor for Direct Detection of Vibrio vulnificus, a Climate Change Biomarker

Autores de IIS La Fe

• María Nieves Aranda Sobrino

  Autor

• María Isabel Caballos Gómez

  Autor

• Alba López Palacios

  Autor

• Elena Aznar Gimeno

  Autor

• Ramón Martínez Máñez

  Autor

• Andy Hernández Montoto

  Autor

Participantes ajenos a IIS La Fe

• Carmona-Salido, H
• Sanjuan, E
• Amaro, C

Grupos

• Unidad Mixta de Investigación en Nanomedicina y Sensores

Abstract

Vibrio vulnificus, a marine pathogen and climate change biomarker, poses serious risks to human and animal health through seafood consumption and seawater exposure. Rapid detection methods are urgently needed for both vibriosis diagnosis and surveillance in warming coastal waters. We report a fluorogenic biosensor based on nanoporous anodic alumina loaded with rhodamine B and capped with an oligonucleotide probe targeting a unique sequence of the vvhA cytolysin gene, specific to V. vulnificus. In the presence of the target DNA, the probe is displaced, pores open, and the fluorophore is released, generating a measurable signal. The biosensor exhibited high sensitivity and selectivity across diverse matrices, including fish mucus and serum, human serum, sterilized brackish water, and—critically—unprocessed natural lake and seawater samples, without DNA extraction or amplification. Detection limits ranged between 10² and 5 × 10² CFU mL?¹, comparable in sensitivity to state-of-the-art qPCR assays. The biosensor outperformed conventional approaches in speed, simplicity, and cost-effectiveness, while maintaining accuracy. These findings underscore the potential of this platform for integrated One Health applications, bridging environmental monitoring with rapid diagnosis of vibriosis in humans and animals. Preliminary results from this study were previously made available as a preprint in SSRN (DOI: https://ssrn.com/abstract=5032822). © 2026 The Author(s). MicrobiologyOpen published by John Wiley & Sons Ltd.

Keywords

• Animals; Biosensing Techniques; Climate Change; Fluorescent Dyes; Humans; Rhodamines; Seawater; Sensitivity and Specificity; Vibrio Infections; Vibrio vulnificus; fluorescent dye; rhodamine; rhodamine B; sea water; animal; climate change; diagnosis; evaluation study; genetic procedures; genetics; human; isolation and purification; metabolism; microbiology; procedures; sensitivity and specificity; veterinary medicine; Vibrio vulnificus; vibriosis