Confined plasmonic device for green and sustainable determination of endogenous H2S emitted by living cells: application to cardiomyocytes

Autores de IIS La Fe

• Marta Gómez Ferrer

  Autor

• Imelda Ontoria Oviedo

  Autor

• Akaitz Dorronsoro González

  Autor

• Sandra Tejedor Gascón

  Autor

• 

  Pilar Sepúlveda Sanchis

  Autor

Participantes ajenos a IIS La Fe

• Hakobyan, L
• Carrero-Ferrer, I
• Ballester-Caudet, A
• Molins-Legua, C
• Campíns-Falcó, P

Grupos

• Regeneración y Trasplante Cardíaco

Abstract

A confined plasmonic colorimetric multisensor adapted microplate format was developed to detect in vitro H2S released by cardiomyocytes. The device is based on nylon membranes spotted with citrate- capped AgNPs and allows configuration of 24, 48 or 96 sensor spots. Upon exposure to H2S at ppb levels, AgNPs change color from yellow to brown as the plasmonic band shifts to higher wavelengths, correlating with H2S concentration. The multisensor and the experimental conditions were optimized to meet the requirements of this biological application including sensitivity, exposure times and cell number. Simulated clinical conditions were tested in AC10 cardiomyocytes and iPSC-derived cardiomyocytes. The method was validated for 24-well plates, achieving a limit of quantification (LOQ) between 0.025 and 0.029 mu g H2S for exposure times ranging from 2 to 20 h, with a relative standard deviation (RSD) below 15%. Satisfactory results were obtained in the determination of H2S released by iPSCs and AC10 cardiomyocytes, with observed levels of approximately 0.4 mu g of H2S. According to these results, it can be concluded that the developed confined multisensor system is suitable for multiple in situ monitoring of H2S released by cardiomyocytes in vitro. Additionally, a quantitative evaluation of sustainability was performed using the hexagon tool. [GRAPHICS] .

Keywords

• Color; Colorimetry; Hydrogen sulfide; Plasmonic nanoparticles; Cardiomyocytes; Colorimetric sensors; Hydrogen sulphie; In-vitro; Living cell; Multi sensor; Multi wells; Multi-well assay; Plasmonic colorimetric sensor; Plasmonics; hydrogen; silver nanoparticle; article; controlled study; diagnosis; drug analysis; human