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Synthetic biology-driven optoelectronic biosensor for rapid and highly sensitive norovirus detection in fecal samples

Fecha de publicación: Fecha Ahead of Print:

Autores de IIS La Fe

Participantes ajenos a IIS La Fe

  • Martorell, S
  • Santiso-Bellón, C
  • Gozalbo-Rovira, R
  • Quintero-Campos, P
  • Luque, D
  • Rodríguez-Díaz, J

Grupos

Abstract

The convergence of synthetic biology and biosensing technologies is revolutionizing diagnostics by enabling highly specific, scalable, and high-throughput detection platforms. In this study, we introduce a synthetic biology-driven optoelectronic biosensor for the rapid and highly sensitive quantification of human norovirus in clinical fecal samples. Our system integrates norovirus-like particles (NoV-LPs), produced via a baculovirusinsect cell expression system, as precise reference nanomaterials, alongside high-affinity single-chain variable fragment (scFv) antibodies generated through phage display as bioreceptors. This novel biosensing platform, implemented on a disc-based system, allows for multiplexed analysis of up to eight specimens within 45 min, eliminating the need for nucleic acid amplification. The sensor provides direct Ct-equivalent values, achieving an exceptional viral detection limit of 32 Ct-equivalent (0.5 fM), demonstrating a 10-to 100-fold improvement in sensitivity over conventional colorimetric biosensors. Clinical validation with 20 human fecal samples confirmed 100 % sensitivity and selectivity, exhibiting a strong correlation with RT-qPCR results (r = 0.945) and a relative error of 11 %. Beyond establishing a highly efficient biosensing platform, this work highlights the transformative impact of synthetic biology to engineer and produce high-affinity bioreceptors for next-generation biosensors with enhanced sensitivity, selectivity, and adaptability across diverse analytical applications.

Copyright © 2025 The Authors. Published by Elsevier B.V. All rights reserved.

Datos de la publicación

ISSN/ISSNe:
0956-5663, 1873-4235

BIOSENSORS & BIOELECTRONICS  ELSEVIER ADVANCED TECHNOLOGY

Tipo:
Article
Páginas:
117787-117787
PubMed:
40674850
Factor de Impacto:
2,113 SCImago ℠
Cuartil:
Q1 SCImago ℠

Citas Recibidas en Web of Science: 4

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Keywords

  • Norovirus GII.4; scFV; VLPs viral infection; Optoelectronic biosensing platform; Global diagnostics

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