Portal de investigación
Oocyte rescue in-vitro maturation does not adversely affect chromosome segregation during the first meiotic division.
Autores de IIS La Fe
Participantes ajenos a IIS La Fe
- Esbert M
- García C
- Cutts G
- Lara-Molina E
- Ballestros A
- Scott RT Jr
- Seli E
- Wells D
Grupos
Abstract
RESEARCH QUESTION: Does rescue in-vitro maturation (IVM) in the presence or absence of cumulus cells, affect the progress of meiosis I, compared with oocytes that mature in vivo? DESIGN: This prospective study was conducted in a university-affiliated fertility centre. Ninety-five young oocyte donors (mean age 25.57 ± 4.47) with a normal karyotype and no known fertility problems were included. A total of 390 oocytes (116 mature metaphase II [MII] and 274 immature oocytes) were analysed. The immature oocytes underwent rescue IVM in the presence of cumulus cells (CC; IVM+CC; n?=?137) or without them (IVM-CC; n?=?137), and IVM rate was calculated. Chromosome copy number analysis using next-generation sequencing (NGS) was performed on all rescue IVM oocytes reaching MII as well as those that were mature at the time of initial denudation (in-vivo-matured oocytes [IVO]). RESULTS: Maturation rates were similar in IVM+CC and IVM-CC oocytes (62.8 versus 71.5%, P?=?0.16). Conclusive cytogenetic results were obtained from 65 MII oocytes from the IVM+CC group, 87 from the IVM-CC group, and 99 from the IVO group. Oocyte euploidy rates for the three groups were similar, at 75.4%, 83.9% and 80.8%, respectively (P?=?0.42). CONCLUSIONS: The results suggest that culture of germinal vesicle and metaphase I oocytes in the presence of cumulus cells does not improve rates of IVM. In general, the process of rescue IVM does not appear to alter the frequency of oocytes with a normal chromosome copy number.
Copyright © 2023 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.
Datos de la publicación
- ISSN/ISSNe:
- 1472-6483, 1472-6491
- Tipo:
- Article
- Páginas:
- 103379-103379
- PubMed:
- 37919136
- Factor de Impacto:
- 1,171 SCImago ℠
- Cuartil:
- Q1 SCImago ℠
REPRODUCTIVE BIOMEDICINE ONLINE ELSEVIER SCI LTD
Citas Recibidas en Web of Science: 10
Documentos
- No hay documentos
Filiaciones
Keywords
- Cumulus cells; Euploidy; In-vitro maturation; Meiosis; Oocyte; Oocyte donor
Cita
Esbert M,García C,Cutts G,Lara E,GARRIDO N,Ballestros A,Scott RTJR,Seli E,Wells D. Oocyte rescue in-vitro maturation does not adversely affect chromosome segregation during the first meiotic division. Reprod. Biomed. Online. 2024. 48. (1):p. 103379-103379. IF:3,500. (1).