Cell-based approaches for the mechanistic understanding of drug-induced cholestatic liver injury.

Autores de IIS La Fe

• Enrique Timor López

  Autor

• 

  Laia Tolosa Pardo

  Autor

• María Teresa Donato Martín

  Autor

Grupos

• Hepatología experimental y Trasplante hepático

• Unidad Mixta de Investigación en Hepatología Experimental

Abstract

Drug-induced cholestasis is one of the major mechanisms implicated in drug-induced hepatotoxicity that poses a serious problem in terms of patient morbidity and mortality, healthcare system expenses and efficacy of newly developed drugs. Impaired bile acid homeostasis due to transporter alterations, hepatocellular injury or canalicular abnormalities is the most characteristic feature of cholestasis. Given the complexity of cholestasis and the different underlying mechanisms, new models and technologies that span a variety of biological processes are needed to accurately predict drugs' cholestatic potential. This review outlines the main triggering mechanisms of drug-induced cholestasis and summarizes the currently available in vitro systems and techniques that attempt to forecast and provide mechanistic details of cholestasis caused by drugs.

© 2025. The Author(s).

Keywords

• Drug-induced cholestasis; In vitro; Mechanisms; Prediction