Co-culture of multiple myeloma cell lines and bone marrow mesenchymal stem cells in a 3D microgel environment.

Autores de IIS La Fe

• Sandra Clara Trujillo

  Autor

• Laia Tolosa Pardo

  Autor

• Lourdes Cordón Gallego

  Autor

• Amparo Sempere Talens

  Autor

Participantes ajenos a IIS La Fe

• Garcia-Briega, M Inmaculada
• Pla-Salom, Julia
• Ribelles, Jose Luis Gomez

Grupos

• Hematología y Hemoterapia

  

  Investigador Principal

  Javier De La Rubia Comos

• Hepatología experimental y Trasplante hepático

  Investigador Principal

  Laia Tolosa Pardo

• Unidad Mixta de Investigación en Hepatología Experimental

  

  Investigador Principal

  Ramiro Jover Atienza

Abstract

This study reproduces the complex relationships between tumour plasma cells and their bone marrow microenvironment in multiple myeloma in vitro. These relationships are established both with other cells and with the extracellular matrix and are key factors in tumour progression, generating resistance to antitumour drugs in the cellular and non-cellular environments. This paper proposes a 3D microenvironment model designed to capture the main components of the multiple myeloma tumour microenvironment. Multiple myeloma cells (MMCs) were dispersed in a microgel medium formed by gel-textured microspheres. The proteins and polysaccharides considered important in the interaction of the MMCs with their non-cellular environment were successfully grafted onto the surface of the microspheres, while human mesenchymal stem cells (MSCs) were cultured in a pellet with non-functionalised microspheres. The MSCs pellet was placed in the well plate together with the microgel and the MMCs and orbitally shaken to maintain them in suspension. The viability, cell cycle and proliferation of the RPMI8226, MM1S and U266 multiple myeloma cell lines and the direct adhesion of MMCs to the MSC pellet were quantified. The results revealed that all three cell lines are able to grow satisfactorily. In addition, the normal behaviour of the MMCs is not modified in any of the culture conditions studied.

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Keywords

• Multiple myeloma; Bone marrow niche; Microgel; Layer by layer; Co-culture; Mesenchymal stem cells; Tissue engineering