Portal de investigación
Detection and Elimination of Senescent Cells with a Self-Assembled Senescence-Associated ß-Galactosidase-Activatable Nanophotosensitizer.
Fecha de publicación:
Fecha Ahead of Print:
Autores de IIS La Fe
Participantes ajenos a IIS La Fe
- Chu JCH
- Xiong J
- Wong CTT
- Wang S
- Tam DY
- García-Fernández A
- Ng DKP
Grupos
Abstract
Senescent cells have become an important therapeutic target for many age-related dysfunctions and diseases. We report herein a novel nanophotosensitizing system that is responsive to the senescence-associated ß-galactosidase (ß-gal) for selective detection and elimination of these cells. It involves a dimeric zinc(II) phthalocyanine linked to a ß-galactose unit via a self-immolative linker. This compound can self-assemble in aqueous media, forming stable nanoscale particles in which the phthalocyanine units are stacked and self-quenched for fluorescence emission and singlet oxygen production. Upon internalization into senescent HeLa cells, these nanoparticles interact with the overproduced senescence-associated ß-gal inside the cells to trigger the disassembly process through enzymatic cleavage of the glycosidic bonds, followed by self-immolation to release the photoactive monomeric phthalocyanine units. These senescent cells can then be lit up with fluorescence and eliminated through the photodynamic action upon light irradiation with a half-maximal inhibitory concentration of 0.06 µM.
Datos de la publicación
- ISSN/ISSNe:
- 0022-2623, 1520-4804
- Tipo:
- Article
- Páginas:
- 234-244
- PubMed:
- 38113190
- Factor de Impacto:
- 1,888 SCImago ℠
- Cuartil:
- Q1 SCImago ℠
JOURNAL OF MEDICINAL CHEMISTRY American Chemical Society
Documentos
- No hay documentos
Filiaciones
Keywords
- PHOTODYNAMIC THERAPY; CELLULAR SENESCENCE; FLUORESCENT-PROBE; PHOTOSENSITIZER; PHTHALOCYANINES; NANOPARTICLES; STRATEGIES; ABLATION
Cita
Chu JCH,Xiong J,Wong CTT,Wang S,Tam DY,García A,MARTÍNEZ R,NGDKP. Detection and Elimination of Senescent Cells with a Self-Assembled Senescence-Associated ß-Galactosidase-Activatable Nanophotosensitizer. J MED CHEM. 2023. 67. (1):p. 234-244. IF:6,800. (1).