Simultaneous Salmonella and bacteriophage isolation on Modified Semisolid Rappaport Vassiliadis media.

Fecha de publicación: 01/10/2023 Fecha Ahead of Print: 25/07/2023

Autores de IIS La Fe

José Miguel Soriano Del Castillo

Autor

Participantes ajenos a IIS La Fe

Torres-Boncompte J
Catalá-Gregori P
Garcia-Llorens J
Sevilla-Navarro S

Grupos

Unidad Mixta de Investigación en Endocrinología, Nutrición y Dietética clínica

Abstract

Salmonella represents a food safety concern worldwide. Despite the application of National Control Programs (NCP) against Salmonella, regulated by the European Union, every year the European Food Safety Authority reports new cases. On the look for new alternatives to antibiotics, bacteriophages, or phages, rise as a promising alternative to treat multidrug resistance infections. Although they are known to be ubiquitous in the environment, their high specificity to host cells hinders their isolation and usage for phage therapy. The ISO 6579-1:2017 is performed as a reference method in the NCP and uses an unspecific media to enrich the sample the same way most phage isolation protocols do. Later, the protocol uses a more selective media to isolate the Salmonella, Modified Semisolid Rappaport Vassiliadis (MSRV). This paper aims to find out whether, due to the similarity between phage isolation protocols and the ISO 6579-1:2017, this last one could be used as a protocol to also isolate phages against the same bacterium that is being simultaneously isolated. To do so, 2 experiments were performed to assess phage isolation from MSRV media in in-vivo conditions. The results from experiments 1 and 2 proved that the MSRV media was usable for simultaneous phage and pathogen isolation through a single procedure. Additionally, there is a correlation between the antigenic formulae from the bacteria and the phage's host range, seeming to be effective against bacteria with similar antigenic formulae.

Datos de la publicación

ISSN/ISSNe:: 0032-5791, 1525-3171
Tipo:: Article
Páginas:: 102960-102960
DOI:: 10.1016/j.psj.2023.102960
PubMed:: 37579648
Enlace a otro recurso:: www.scopus.com
Factor de Impacto:: 0,982 SCImago ℠
Cuartil:: Q1 SCImago ℠

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Filiaciones

Torres-Boncompte J:: Food & Health Lab, Institute of Materials Science, University of Valencia, Valencia, 46980, Spain

Centro de Calidad Avícola y Alimentación Animal de la Comunidad Valenciana (CECAV), Castellón, 12539, Spain
Catalá-Gregori P:: Centro de Calidad Avícola y Alimentación Animal de la Comunidad Valenciana (CECAV), Castellón, 12539, Spain

Departamento de Producción y Sanidad Animal, Salud Pública Veterinaria y Ciencia y Tecnología de los Alimentos, Instituto de Ciencias Biomédicas, Facultad de Veterinaria, Universidad Cardenal Herrera-CEU, CEU Universities, Moncada, 46113, Spain
Garcia-Llorens J:: Food & Health Lab, Institute of Materials Science, University of Valencia, Valencia, 46980, Spain

Centro de Calidad Avícola y Alimentación Animal de la Comunidad Valenciana (CECAV), Castellón, 12539, Spain
Soriano JM:: Food & Health Lab, Institute of Materials Science, University of Valencia, Valencia, 46980, Spain

Instituto de Investigación. Joint Research Unit on Endocrinology, Nutrition and Clinical Dietetics, University of Valencia-Health Research Institute La Fe, Valencia, 46026, Spain
Sevilla-Navarro S:: Centro de Calidad Avícola y Alimentación Animal de la Comunidad Valenciana (CECAV), Castellón, 12539, Spain

Departamento de Producción y Sanidad Animal, Salud Pública Veterinaria y Ciencia y Tecnología de los Alimentos, Instituto de Ciencias Biomédicas, Facultad de Veterinaria, Universidad Cardenal Herrera-CEU, CEU Universities, Moncada, 46113, Spain

Keywords

Rappaport; Salmonella; bacteriophages; isolation; poultry

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