Portal de investigación
ß-Galactosidase-Activatable Nile Blue-Based NIR Senoprobe for the Real-Time Detection of Cellular Senescence.
Fecha de publicación:
Fecha Ahead of Print:
Autores de IIS La Fe
Participantes ajenos a IIS La Fe
- Lozano-Torres, Beatriz
- Garcia-Fernandez, Alba
- Dominguez, Marcia
- Blandez, Juan F.
Grupos
Abstract
Cellular senescence is a stable cell cycle arrest in response to stress or other damage stimuli to maintain tissue homeostasis. However, the accumulation of senescent cells can lead to the progression of various senescence-related disorders. In this paper, we describe the development of a ß-galactosidase-activatable near-infrared (NIR) senoprobe, NBGal, for the detection of senescent cells based on the use of the FDA-approved Nile blue (NB) fluorophore. NBGal was validated in chemotherapeutic-induced senescence cancer models in vitro using SK-Mel 103 and 4T1 cell lines. In vivo monitoring of cellular senescence was evaluated in orthotopic triple-negative breast cancer-bearing mice treated with palbociclib to induce senescence. In all cases, NBGal exhibited a selective tracking of senescent cells mainly ascribed to the overexpressed ß-galactosidase enzyme responsible for hydrolyzing the NBGal probe generating the highly emissive NB fluorophore. In this way, NBGal has proven to be a qualitative, rapid, and minimally invasive probe that allows the direct detection of senescent cells in vivo.
Datos de la publicación
- ISSN/ISSNe:
- 0003-2700, 1520-6882
- Tipo:
- Article
- Páginas:
- 1643-1651
- PubMed:
- 36580602
- Factor de Impacto:
- 1,791 SCImago ℠
- Cuartil:
- Q1 SCImago ℠
ANALYTICAL CHEMISTRY AMER CHEMICAL SOC
Citas Recibidas en Web of Science: 1
Documentos
- No hay documentos
Filiaciones
Keywords
- 2-PHOTON FLUORESCENT-PROBE; CANCER; BIOMARKER; CELLS
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Cita
Lozano B,Garcia A,Dominguez M,SANCENON F,Blandez JF,MARTINEZ R. ß-Galactosidase-Activatable Nile Blue-Based NIR Senoprobe for the Real-Time Detection of Cellular Senescence. Anal Chem. 2022. 95. (2):p. 1643-1651. IF:7,400. (1).