Concentration-Dependent Protection by Ethanol Extract of Propolis against ?-Ray-Induced Chromosome Damage in Human Blood Lymphocytes.

Autores de IIS La Fe

• Joan Francesc Barquinero Estruch

  Autor

• Miguel Jose Almonacid Bujeda

  Autor

• Alegría Montoro Pastor

  Autor

• Natividad Sebastiá Fabregat

  Autor

• Gumersindo Jesus Verdu Martin

  Autor

• Juan Serrano Gandia

  Autor

• Juan Ignacio Villaescusa Blanca

  Autor

• José Miguel Soriano Del Castillo

  Autor

Participantes ajenos a IIS La Fe

• Sahuquillo V
• Saiz M

Abstract

Radioprotection with natural products may be relevant to the mitigation of ionizing radiation-induced damage in mammalian systems; in this sense, propolis extracts have shown effects such as antioxidant, antitumoral, anti-inflammatory, and immunostimulant. We report for the first time a cytogenetic study to evaluate the radioprotective effect, in vitro, of propolis against radiation-induced chromosomal damage. Lymphocytes were cultured with increasing concentrations of ethanol extract of propolis (EEP), including 20, 40, 120, 250, 500, 750, 1000, and 2000 µg mL(-1) and then exposed to 2 Gy ?-rays. A significant and concentration-dependent decrease is observed in the frequency of chromosome aberrations in samples treated with EEP. The protection against the formation of dicentrics was concentration-dependent, with a maximum protection at 120 µg mL(-1) of EEP. The observed frequency of dicentrics is described as negative exponential function, indicating that the maximum protectible fraction of dicentrics is approximately 44%. Free radical scavenging and antioxidant activities are the mechanisms that these substances use to protect cells from ionizing radiation.