Human Milk Bactericidal Properties: Effect of Lyophilization and Relation to Maternal Factors and Milk Components
Autores de IIS La Fe
Participantes ajenos a IIS La Fe
- Salcedo J
- López-Mendoza MC
- Nogarotto E
- Silvestre D
Grupos
Abstract
Objective: Lyophilization appears to be a viable method for storing human milk, assuring no microbiological contamination and preserving its health benefits and antibacterial properties. The aim of the study is to evaluate and compare the effects of different storage methods (lyophilization and freezing at -20 degrees C and -80 degrees C) and maternal factors (gestational length or time postpartum) upon the microbiological contents and bactericidal activity of human milk. The possible relation between bactericidal activity and the content of certain nutrients and functional components is also investigated. Methods: Microbiological content, bactericidal activity, sialic acid, and ganglioside contents, as well as protein, fat, and lactose concentrations were assessed in 125 human milk samples from 65 healthy donors in the Human Milk Bank of La Fe (Valencia, Spain). Results: Lyophilization and storage at -80 degrees C significantly reduced the content of mesophilic aerobic microorganisms and Staphylococcus epidermidis when compared with storage at -20 degrees C. Bactericidal activity was not significantly modified by lyophilization when compared with freezing at either -20 degrees C or -80 degrees C. Bactericidal activity was not correlated with fat, protein, or lactose content, but was significantly correlated to ganglioside content. The bactericidal activity was significantly greater (P<0.05) in mature milk and in milk from women with term delivery than in milk from early lactation (days 1-7 postpartum) and milk from women with preterm delivery, respectively. Conclusions: Lyophilization and storage at -80 degrees C of human milk yields similar results and are superior to storage at -20C with regard to microbial and bactericidal capacities, being a feasible alternative for human milk banks.
Datos de la publicación
- ISSN/ISSNe:
- 0277-2116, 1536-4801
- Tipo:
- Article
- Páginas:
- 527-532
- PubMed:
- 25406523
- Factor de Impacto:
- 1,239 SCImago ℠
- Cuartil:
- Q1 SCImago ℠
JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION LIPPINCOTT WILLIAMS & WILKINS
Citas Recibidas en Web of Science: 20
Documentos
- No hay documentos
Filiaciones
Keywords
- bactericidal activity; breast-feeding; human milk; human milk bank; lyophilization
Proyectos y Estudios Clínicos
PROTECCION FRENTE AL ESTRES OXIDATIVO DE LOS PREMATUROS ALIMENTADOS CON LECHE MATERNA DONADA PROCESADA EN EL BANCO DE LECHE DEL HOSPITAL LA FE
Investigador Principal: MARÍA JOSÉ GORMAZ MORENO
GE-006/10 . 2010
ALTERACIONES METABONOMICAS EN EL PREMATURO EXTREMO DEPENDIENTES DE LA CARGA DE OXIGENO RECIBIDA EN LA REANIMACION POSTNATAL: UN ESTUDIO PROSPECTIVO ALEATORIZADO.
Investigador Principal: MÁXIMO VENTO TORRES
PI11/00313 . INSTITUTO DE SALUD CARLOS III . 2012
RED DE SALUD MATERNO INFANTIL Y DEL DESARROLLO
Investigador Principal: MÁXIMO VENTO TORRES
RD12/0026/0012 . INSTITUTO DE SALUD CARLOS III; FUNDACIÓN PARA LA INVESTIGACIÓN DEL HOSPITAL UNIVERSITARIO LA FE DE LA COMUNIDAD VALENCIANA . 2013
Cita
Salcedo J,GORMAZ M,López MC,Nogarotto E,Silvestre D. Human Milk Bactericidal Properties: Effect of Lyophilization and Relation to Maternal Factors and Milk Components. J Pediatr Gastroenterol Nutr. 2015. 60. (4):p. 527-532. IF:2,400. (1).