Isolation and Lipidomic Screening of Human Milk Extracellular Vesicles.

Fecha de publicación: 01/01/2023

Autores de IIS La Fe

Victoria Ramos García

Autor
Isabel Ten Doménech

Autor
Abel Albiach Delgado

Autor
Marta Gómez Ferrer

Autor
Pilar Sepúlveda Sanchis

Autor
Anna Parra Llorca

Autor
Laura Campos Berga

Autor
Alba Moreno Giménez

Autor
Guillermo Quintas Soriano

Autor
Julia Kuligowski

Autor

Grupos

Investigación en Salud Mental

Leader

Ana Cristina García Blanco
Perinatología

Leader

Máximo Vento Torres
Regeneración y Trasplante Cardíaco

Leader

Pilar Sepúlveda Sanchis

Abstract

Extracellular vesicles (EVs) are secreted by cells and can be found in biological fluids (e.g., blood, saliva, urine, cerebrospinal fluid, and milk). EV isolation needs to be optimized carefully depending on the type of biofluid and tissue. Human milk (HM) is known to be a rich source of EVs, and they are thought to be partially responsible for the benefits associated with breastfeeding. Here, a workflow for the isolation and lipidomic analysis of HM-EVs is described. The procedure encompasses initial steps such as sample collection and storage, a detailed description for HM-EV isolation by multistage ultracentrifugation, metabolite extraction, and analysis by liquid chromatography coupled to mass spectrometry, as well as data analysis and curation.

Datos de la publicación

ISSN/ISSNe:: 1064-3745, 1940-6029
Tipo:: Article
Páginas:: 177-188
DOI:: 10.1007/978-1-0716-2699-3_18
Factor de Impacto:: 0,368 SCImago ℠
Cuartil:: Q4 SCImago ℠

Documentos

No hay documentos

Métricas

Filiaciones

Ramos-Garcia, Victoria:: Instituto de Investigación. Neonatal Research Group, Health Research Institute La Fe, Valencia, Spain
Ten-Domenech, Isabel:: Instituto de Investigación. Neonatal Research Group, Health Research Institute La Fe, Valencia, Spain
Albiach-Delgado, Abel:: Instituto de Investigación. Neonatal Research Group, Health Research Institute La Fe, Valencia, Spain
Gomez-Ferrer, Marta:: Regenerative Medicine and Heart Transplantation Unit, Health Research Institute La Fe, Valencia, Spain
Sepulveda, Pilar:: Regenerative Medicine and Heart Transplantation Unit, Health Research Institute La Fe, Valencia, Spain
Parra-Llorca, Anna:: Instituto de Investigación. Neonatal Research Group, Health Research Institute La Fe, Valencia, Spain
Campos-Berga, Laura:: Instituto de Investigación. Neonatal Research Group, Health Research Institute La Fe, Valencia, Spain
Moreno-Gimenez, Alba:: Instituto de Investigación. Neonatal Research Group, Health Research Institute La Fe, Valencia, Spain
Quintas, Guillermo:: Health and Biomedicine, Leitat Technological Center, Terrassa, Spain

Instituto de Investigación. Analytical Unit, Health Research Institute La Fe, Valencia, Spain
Kuligowski, Julia:: Instituto de Investigación. Neonatal Research Group, Health Research Institute La Fe, Valencia, Spain.

Keywords

Exosomes; Extracellular vesicles; Human milk (HM); Lipidomics; Liquid chromatography–mass spectrometry (LC–MS)

Proyectos asociados

CREACION Y MEJORA DE INFRAESTRUCTURAS EN MEDICINA REGENERATIVA

Investigador Principal: FUNDACIÓN PARA LA INVESTIGACIÓN DEL HOSPITAL UNIVERSITARIO LA FE DE LA COMUNIDAD VALENCIANA

PLE2009-0141 ACI-PLAN E . MINISTERIO DE CIENCIA E INNOVACION; FUNDACIÓN PARA LA INVESTIGACIÓN DEL HOSPITAL UNIVERSITARIO LA FE DE LA COMUNIDAD VALENCIANA . 2009