Ultra high performance liquid chromatography coupled to tandem mass spectrometry determination of lipid peroxidation biomarkers in newborn serum samples

Fecha de publicación: 30/07/2015

Autores de IIS La Fe

Consuelo Cháfer Pericás

Autor
Ángel Sánchez Illana

Autor
Julia Kuligowski

Autor
María Isabel Torres Cuevas

Autor
María Cernada Badía

Autor
Elena Cubells Garcia

Autor
Antonio Guillermo Núñez Ramiro

Autor
Máximo Vento Torres

Autor
Justo Javier Escobar Cubiella

Autor

Participantes ajenos a IIS La Fe

Rahkonen L
Andersson S

Grupos

Perinatología

Leader

Máximo Vento Torres

Abstract

Byproducts of arachidonic (AA) and docosahexaenoic acid (DHA) oxidation are highly relevant for the study of free radical associated conditions in the perinatal period. Plasma metabolites can provide the clinician with a snapshot of the oxidant status of patients before and after specific clinical interventions (e.g.: supplementation with oxygen). We describe a new andreliable ultra-performance liquid mass spectrometry method to determine F2-isoprostanes and other byproducts (isoprostanes, isofurans, neuroprostanes, neurofurans) in newborn serum samples. Cord blood samples were obtained from severely depressed newborn infants (Apgar score 1 min < 3; arterial cord pH < 7.00), and aliquoted for serum determination and stored at -80 degrees C. AUHPLC-MS/MS method was employed. It has a series of technical advantages: simple sample treatment; reduced sample volume (100 mu L) which is essential for preterm neonates with low circulating blood volume, high throughput of sample analysis (96 samples in less than 24 h) and high selectivity for different isoprostanes isomers. Excellent sensitivity was achieved within limits of detection between 0.06 and 4.2 nmol L-1, which renders this method suitable to monitoranalyte concentration in newborn samples. The method's precision was satisfactory; with coefficients of variation around 5-12% (intra-day) and 7-17% (inter-day). The reliability of the described method was assessed by analysis of spiked serum samples obtaining recoveries between 70% and 120%. The proposed method has rendered suitable for serum determination for newborn babies at risk of oxygen free radical associated conditions. (C) 2015 Elsevier B.V. All rights reserved.

Datos de la publicación

ISSN/ISSNe:: 0003-2670, 1873-4324
Tipo:: Article
Páginas:: 214-220
DOI:: 10.1016/j.aca.2015.06.028
Factor de Impacto:: 1,469 SCImago ℠
Cuartil:: Q1 SCImago ℠

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Filiaciones

Cháfer-Pericás C:: Instituto de Investigación. Neonatal Research Group, Health Research Institute La Fe, Valencia, Spain
Rahkonen L:: Department of Obstetrics and Gynecology, University of Helsinki, Helsinki University Hospital, Helsinki, Finland
Sánchez-Illana A:: Instituto de Investigación. Neonatal Research Group, Health Research Institute La Fe, Valencia, Spain
Kuligowski J:: Instituto de Investigación. Neonatal Research Group, Health Research Institute La Fe, Valencia, Spain
Torres-Cuevas I:: Instituto de Investigación. Neonatal Research Group, Health Research Institute La Fe, Valencia, Spain
Cernada M:: Division of Neonatology, University & Polytechnic Hospital La Fe, Valencia, Spain
Cubells E:: Division of Neonatology, University & Polytechnic Hospital La Fe, Valencia, Spain
Nuñez-Ramiro A:: Division of Neonatology, University & Polytechnic Hospital La Fe, Valencia, Spain
Andersson S:: Children's Hospital, University of Helsinki, Helsinki University Hospital, Helsinki, Finland
Vento M:: Division of Neonatology, University & Polytechnic Hospital La Fe, Valencia, Spain
Escobar J:: Instituto de Investigación. Neonatal Research Group, Health Research Institute La Fe, Valencia, Spain.