ATR-Spin: an open-source 3D printed device for direct cytocentrifugation onto attenuated total reflectance crystals.

Autores de IIS La Fe

• Guillermo Quintas Soriano

  Autor

• Julia Kuligowski

  Autor

Participantes ajenos a IIS La Fe

• Pérez-Guaita D
• Richardson Z
• Bedolla DE
• Byrne HJ
• Wood B

Grupos

• Perinatología

  

  Leader

  Máximo Vento Torres

Abstract

Infrared spectroscopy (IR) enables the direct and rapid characterization of cells at the molecular level. Achieving a rapid and consistent cell preparation is critical for the development of point-of-care diagnostics for cell analysis. Here we introduce an open-source, 3D printed device for integrating the isolation, preconcentration, and measurement of attenuated total reflectance IR spectra of cells from biofluids. The tool comprises a disposable card for cytocentrifugation, equipped with magnets, which allows reproducible integration into the pathlength of the IR spectrophotometer. Preliminary results using cell culture media containing A549 cells indicate that this system enables a qualitative and quantitative characterization of cells down to 10 cells µL(-1) by using a single and cost-effective device and within a few minutes.

Keywords

• URINE; POINT; SERUM