Metabolomic Analysis of the Effect of Postnatal Hypoxia on the Retina in a Newly Born Piglet Model.

Autores de IIS La Fe

• Justo Javier Escobar Cubiella

  Autor

• María Isabel Torres Cuevas

  Autor

• 

  Agustín Lahoz Rodríguez

  Autor

• 

  Máximo Vento Torres

  Autor

• Julia Kuligowski

  Autor

Participantes ajenos a IIS La Fe

• Solberg R
• Arduini A
• Sastre J
• Saugstad OD
• Quintás G

Grupos

• Perinatología

  

  Leader

  Máximo Vento Torres

Abstract

The availability of reliable biomarkers of brain injury secondary to birth asphyxia could substantially improve clinical grading, therapeutic intervention strategies, and prognosis. In this study, changes in the metabolome of retinal tissue caused by profound hypoxia in an established neonatal piglet model were investigated using an ultra performance liquid chromatography - quadrupole time of flight mass spectrometry (UPLC-QTOFMS) untargeted metabolomic approach, which included Partial Least Squares - Discriminant Analysis (PLSDA) multivariate data analysis. The initial identification of a set of discriminant metabolites from UPLC-QTOFMS data was confirmed by target UPLC-MS/MS and allowed the selection of endogenous CDP-choline as a promising candidate biomarker for hypoxia-derived brain damage assessing intensity of retinal hypoxia. Results from this study will foster further research on CDP-choline changes occurring during resuscitation.