Portal de investigación
Crohn's Disease Increases the Mesothelial Properties of Adipocyte Progenitors in the Creeping Fat
Fecha de publicación:
Fecha Ahead of Print:
Autores de IIS La Fe
Participantes ajenos a IIS La Fe
- Madeira, A
- Serena, C
- Ejarque, M
- Maymo-Masip, E
- Navarro-Ruiz, MC
- Guzman-Ruiz, R
- Malagon, MM
- Espin, E
- Marti, M
- Menacho, M
- Megia, A
- Vendrell, J
- Fernandez-Veledo, S
Grupos
Abstract
Our understanding of the interplay between human adipose tissue and the immune system is limited. The mesothelium, an immunologically active structure, emerged as a source of visceral adipose tissue. After investigating the mesothelial properties of human visceral and subcutaneous adipose tissue and their progenitors, we explored whether the dysfunctional obese and Crohn's disease environments influence the mesothelial/mesenchymal properties of their adipocyte precursors, as well as their ability to mount an immune response. Using a tandem transcriptomic/proteomic approach, we evaluated the mesothelial and mesenchymal expression profiles in adipose tissue, both in subjects covering a wide range of body-mass indexes and in Crohn's disease patients. We also isolated adipose tissue precursors (adipose-derived stem cells, ASCs) to assess their mesothelial/mesenchymal properties, as well as their antigen-presenting features. Human visceral tissue presented a mesothelial phenotype not detected in the subcutaneous fat. Only ASCs from mesenteric adipose tissue, named creeping fat, had a significantly higher expression of the hallmark mesothelial genes mesothelin (MSLN) and Wilms' tumor suppressor gene 1 (WT1), supporting a mesothelial nature of these cells. Both lean and Crohn's disease visceral ASCs expressed equivalent surface percentages of the antigen-presenting molecules human leucocyte antigen-DR isotype (HLA-DR) and CD86. However, lean-derived ASCs were predominantly HLA-DR (dim), whereas in Crohn's disease, the HLA-DR (bright) subpopulation was increased 3.2-fold. Importantly, the mesothelial-enriched Crohn's disease precursors activated CD4(+) T-lymphocytes. Our study evidences a mesothelial signature in the creeping fat of Crohn's disease patients and its progenitor cells, the latter being able to present antigens and orchestrate an immune response.
Datos de la publicación
- ISSN/ISSNe:
- 1661-6596, 1661-6596
- Tipo:
- Article
- Páginas:
- -
- DOI:
- 10.3390/ijms22084292
- PubMed:
- 33924264
- Factor de Impacto:
- 1,176 SCImago ℠
- Cuartil:
- Q1 SCImago ℠
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES MDPI AG
Citas Recibidas en Web of Science: 4
Documentos
- No hay documentos
Filiaciones
Keywords
- Crohn’ s disease; adipose tissue; mesothelium; adipose-derived stem cells
Proyectos y Estudios Clínicos
THE MOLECULAR SIGNATURE AND ITS FUNCTIONAL RELEVANCE IN PENETRATING VS STENOTIC CROHN´S DISEASE.
Investigador Principal: MARIA DOLORES BARRACHINA SANCHO
PID2019-108996RB-I00 . MINISTERIO DE CIENCIA E INNOVACION . 2020
ESTUDIO DE SEGURIDAD POSAUTORIZACIÓN PARA EVALUAR LA SEGURIDAD Y LA EFICACIA A LARGO PLAZO DE LA ADMINISTRACIÓN REPETIDA DE DARVADSTROCEL EN PACIENTES CON ENFERMEDAD DE CROHN Y FÍSTULAS PERIANALES COMPLEJAS (ASPIRE).
Investigador Principal: ADELA BATISTA DOMENECH
ALOFISEL-4001 . 2020
ESTUDIO MULTICÉNTRICO PROSPECTIVO, CONTROLADO, ALEATORIZADO DE NO INFERIORIDAD SOBRE EL MANEJO DE LA SONDA VESICAL EN CIRUGÍA DE COLON.
Investigador Principal: MÓNICA MILLÁN SCHEIDING
CR-VESICATH . 2021
Cita
Madeira A,Serena C,Ejarque M,Maymo E,MILLAN M,Navarro MC,Guzman R,Malagon MM,Espin E,Marti M,Menacho M,Megia A,Vendrell J,Fernandez S. Crohn's Disease Increases the Mesothelial Properties of Adipocyte Progenitors in the Creeping Fat. Int. J. Mol. Sci. 2021. 22. (8):4292. IF:6,208. (1).