Assessment in vitro of cytogenetic and genotoxic effects of propolis on human lymphocytes.

Autores de IIS La Fe

• Alegría Montoro Pastor

  Autor

• José Miguel Soriano Del Castillo

  Autor

• Joan Francesc Barquinero Estruch

  Autor

• Miguel Jose Almonacid Bujeda

  Autor

• Juan Ignacio Villaescusa Blanca

  Autor

• Natividad Sebastiá Fabregat

  Autor

Participantes ajenos a IIS La Fe

• Verdú G
• Sahuquillo V

Abstract

We evaluated the genetic damage by ethanolic extract of propolis (EEP) induced to human lymphocytes which were exposed to increasing concentrations (0-2000µgml(-1)). The results indicated that EEP reduced significantly the mitotic index (MI) and proliferation index (PI) when high concentrations of EEP were used. Sister chromatid exchange (SCE) rates indicated that EEP could have genotoxic effects at high concentrations. Exposure of the cells to the amount of ethanol used as solvent did not alter either the MI and cell proliferation kinetics (CPK), or the rate of SCE. The results showed: (a) statistical increase in the percentage the cells with CAs and in the frequency of SCE at the highest concentrations, (b) a decrease in MI and in the CPK values was observed, (c) no effect was noticed in negative controls. In conclusion, it can be assumed that high concentrations of EEP have a cyto and genotoxic effect, in vitro, for human peripheral lymphocytes.