Radioprotective activity and cytogenetic effect of resveratrol in human lymphocytes: an in vitro evaluation.

Autores de IIS La Fe

• Natividad Sebastiá Fabregat

  Autor

• Miguel Jose Almonacid Bujeda

  Autor

• Juan Ignacio Villaescusa Blanca

  Autor

• José Vicente Cervera Zamora

  Autor

• Esperanza Such Taboada

  Autor

• María Angeles Silla Company

  Autor

• José Miguel Soriano Del Castillo

  Autor

• Alegría Montoro Pastor

  Autor

Grupos

• Hematología y Hemoterapia

  

  Investigador Principal

  Javier De La Rubia Comos

• Investigacion Biomédica en Imagen (GIBI 2-30)

  Investigador Principal

  Leonor Cerdá Alberich

Abstract

Trans-resveratrol is a natural occurring polyphenol, obtained from grapes and other berries. This compound has shown antioxidant, anti-inflammatory, immunostimulant or anti-carcinogenic properties. Our aim was to evaluate the radioprotective efficacy, in vitro, of trans-resveratrol against radiation-induced chromosomal damage and to study the genotoxicity and cytotoxicity of this polyphenol in cell cultures without irradiation. The study was carried out by the pre-treatment of human lymphocytes at concentrations from 0 to 219µM of trans-resveratrol. The results showed that all concentrations tested reduced radiation-induced chromosomal damage compared with cells with any treatment. Maximum damage protection was observed at the concentration of 2.19µM. Concerning genotoxic results, all tested trans-resveratrol concentrations increased the sister chromatid exchange (SCE) index compared with no trans-resveratrol treatment. Cytotoxic indexes (Mitotic and Proliferation Index) showed that the lowest concentrations could enhance the cell proliferation rates and the highest ones could negatively affect to human peripheral lymphocytes growth.