Portal de investigación
A Two-Photon Probe Based on Naphthalimide-Styrene Fluorophore for the In Vivo Tracking of Cellular Senescence.
Fecha de publicación:
Fecha Ahead of Print:
Autores de IIS La Fe
Participantes ajenos a IIS La Fe
- Lozano-Torres, Beatriz
- Blandez, Juan F.
- Galiana, Irene
- Lopez-Dominguez, Jose A.
- Rovira, Miguel
- Paez-Ribes, Marta
- Gonzalez-Gualda, Estela
- Muñoz-Espín D
- Serrano, Manuel
- Sancenon, Felix
Grupos
Abstract
Cellular senescence is a state of stable cell cycle arrest that can negatively affect the regenerative capacities of tissues and can contribute to inflammation and the progression of various aging-related diseases. Advances in the in vivo detection of cellular senescence are still crucial to monitor the action of senolytic drugs and to assess the early onset or accumulation of senescent cells. Here, we describe a naphthalimide-styrene-based probe ( HeckGal ) for the detection of cellular senescence both in vitro and in vivo . HeckGal is hydrolyzed by the increased lysosomal ß-galactosidase activity of senescent cells, resulting in fluorescence emission. The probe was validated in vitro using normal human fibroblasts and various cancer cell lines undergoing senescence induced by different stress stimuli. Remarkably, HeckGal was also validated in vivo in an orthotopic breast cancer mouse model treated with senescence-inducing chemotherapy and in a renal fibrosis mouse model. In all cases, HeckGal allowed the unambiguous detection of senescence in vitro as well as in tissues and tumors in vivo . This work is expected to provide a potential technology for senescence detection in aged or damaged tissues.
Datos de la publicación
- ISSN/ISSNe:
- 0003-2700, 1520-6882
- Tipo:
- Article
- Páginas:
- 3052-3060
- Factor de Impacto:
- 1,791 SCImago ℠
- Cuartil:
- Q1 SCImago ℠
ANALYTICAL CHEMISTRY AMER CHEMICAL SOC
Citas Recibidas en Web of Science: 18
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- No hay documentos
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Cita
Lozano B,Blandez JF,Galiana I,Lopez JA,Rovira M,Paez M,Gonzalez E,Muñoz D,Serrano M,Sancenon F,Martinez R. A Two-Photon Probe Based on Naphthalimide-Styrene Fluorophore for the In Vivo Tracking of Cellular Senescence. Anal Chem. 2021. 93. (5):p. 3052-3060. IF:8,008. (1).