RNA-sequencing analysis reveals new alterations in cardiomyocyte cytoskeletal genes in patients with heart failure

Autors de IIS La Fe

• Isabel Herrer Mambrona

  Autor

• 

  Esther Roselló Lletí

  Autor

• Jose Miguel Rivera Otero

  Autor

• María Micaela Molina Navarro

  Autor

• Estefanía Tarazón Melguizo

  Autor

• Ana Ortega Gutierrez

  Autor

• 

  Luis Vicente Martínez Dolz

  Autor

• Carlos Gonzalez Juanatey

  Autor

• Vicente Bertomeu Martinez

  Autor

• José Anastasio Montero Argudo

  Autor

• Manuel Portoles Sanz

  Autor

Autors aliens a IIS La Fe

• Triviño JC
• Lago F

Grups d'Investigació

• Grupo de Investigación Clínica y Traslacional en Cardiología

  

  Investigador Principal

  Esther Roselló Lletí

• Regeneración y Trasplante Cardíaco

  

  Leader

  Pilar Sepúlveda Sanchis

Abstract

Changes in cardiomyocyte cytoskeletal components, a crucial scaffold of cellular structure, have been found in heart failure (HF); however, the altered cytoskeletal network remains to be elucidated. This study investigated a new map of cytoskeleton-linked alterations that further explain the cardionnyocyte morphology and contraction disruption in HF. RNA-Sequencing (RNA-Seq) analysis was performed in 29 human LV tissue samples from ischemic cardiomyopathy (ICM; n = 13) and dilated cardiomyopathy (DCM, n = 10) patients undergoing cardiac transplantation and six healthy donors (control, CNT) and up to 16 ICM, 13 DCM and 7 CNT tissue samples for qRT-PCR. Gene Ontology analysis of RNA-Seq data demonstrated that cytoskeletal processes are altered in HF. We identified 60 differentially expressed cytoskeleton-related genes in ICM and 58 genes in DCM comparing with CNT, hierarchical clustering determined that shared cytoskeletal genes have a similar behavior in both pathologies. We further investigated MYLK4, RHOU, and ANKRD1 cytoskeletal components. qRT-PCR analysis revealed that MYLK4 was downregulated ( - 2.2-fold; P < 0.05) and ANKRD1 was upregulated (2.3-fold; P < 0.01) in ICM patients vs CNT. RHOU mRNA levels showed a statistical trend to decrease ( 2.9-fold). In DCM vs CNT, MYLK4 ( - 4.0-fold; P < 0.05) and RHOU ( - 3.9-fold; P < 0.05) were downregulated and ANKRD1 (2.5-fold; P < 0.05) was upregulated. Accordingly, MYLK4 and ANKRD1 protein levels were decreased and increased, respectively, in both diseases. Furthermore, ANKRD1 and RHOU mRNA levels were related with LV function (P < 0.05). In summary, we have found a new map of changes in the ICM and DCM cardiomyocyte cytoskeleton. ANKRD1 and RHOU mRNA levels were related with LV function which emphasizes their relevance in HF. These new cytoskeletal changes may be responsible for altered contraction and cell architecture disruption in HF patients. Moreover, these results improve our knowledge on the role of cytoskeleton in functional and structural alterations in HF.

Keywords

• ANKRD1; cytoskeleton; heart failure; left ventricular function; MYLK4; RHOU; RNA-Seq