Intracellular role of IL-6 in mesenchymal stromal cell immunosuppression and proliferation.

Fecha de publicación: 14/12/2020 Fecha Ahead of Print: 14/12/2020

Autores de IIS La Fe

Akaitz Dorronsoro González

Autor
Pilar Sepúlveda Sanchis

Autor

Participantes ajenos a IIS La Fe

Lang, Valerie
Ferrin, Izaskun
Fernandez-Rueda, Jon
Zabaleta, Lorea
Perez-Ruiz, Estibaliz
Trigueros, Cesar

Grupos

Regeneración y Trasplante Cardíaco

Leader

Pilar Sepúlveda Sanchis

Abstract

Interleukin (IL)-6 is a pleiotropic cytokine involved in the regulation of hematological and immune responses. IL-6 is secreted chiefly by stromal cells, but little is known about its precise role in the homeostasis of human mesenchymal stromal cells (hMSCs) and the role it may play in hMSC-mediated immunoregulation. We studied the role of IL-6 in the biology of bone marrow derived hMSC in vitro by silencing its expression using short hairpin RNA targeting. Our results show that IL-6 is involved in immunosuppression triggered by hMSCs. Cells silenced for IL-6 showed a reduced capacity to suppress activated T-cell proliferation. Moreover, silencing of IL-6 significantly blocked the capacity of hMSCs to proliferate. Notably, increasing the intracellular level of IL-6 but not recovering the extracellular level could restore the proliferative impairment observed in IL-6-silenced hMSC. Our data indicate that IL-6 signals in hMSCs by a previously undescribed intracellular mechanism.

Datos de la publicación

ISSN/ISSNe:: 2045-2322, 2045-2322
Tipo:: Article
Páginas:: 21853-21853
DOI:: 10.1038/s41598-020-78864-4
Factor de Impacto:: 1,240 SCImago ℠
Cuartil:: Q1 SCImago ℠

Documentos

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Métricas

Filiaciones

Dorronsoro, Akaitz:: [ 1 ]? Fdn Stem Cell Res, Fdn Inbiomed, Mesenchymal Stem Cell Lab, Paseo Mikeletegi 81, San Sebastian 20009, Spain

Instituto de Investigación. [ 2 ]? Inst Invest Sanitaria La Fe, Regenerat Med & Heart Transplantat Unit, Ave Fernando Abril Martorell 106,Torre A,Lab 5-04, Valencia 46026, Spain
Lang, Valerie:: [ 1 ]? Fdn Stem Cell Res, Fdn Inbiomed, Mesenchymal Stem Cell Lab, Paseo Mikeletegi 81, San Sebastian 20009, Spain
Ferrin, Izaskun:: [ 1 ]? Fdn Stem Cell Res, Fdn Inbiomed, Mesenchymal Stem Cell Lab, Paseo Mikeletegi 81, San Sebastian 20009, Spain
Fernandez-Rueda, Jon:: [ 1 ]? Fdn Stem Cell Res, Fdn Inbiomed, Mesenchymal Stem Cell Lab, Paseo Mikeletegi 81, San Sebastian 20009, Spain
Zabaleta, Lorea:: [ 1 ]? Fdn Stem Cell Res, Fdn Inbiomed, Mesenchymal Stem Cell Lab, Paseo Mikeletegi 81, San Sebastian 20009, Spain
Perez-Ruiz, Estibaliz:: [ 1 ]? Fdn Stem Cell Res, Fdn Inbiomed, Mesenchymal Stem Cell Lab, Paseo Mikeletegi 81, San Sebastian 20009, Spain
Sepulveda, Pilar:: Instituto de Investigación. [ 2 ]? Inst Invest Sanitaria La Fe, Regenerat Med & Heart Transplantat Unit, Ave Fernando Abril Martorell 106,Torre A,Lab 5-04, Valencia 46026, Spain
Trigueros, Cesar:: [ 1 ]? Fdn Stem Cell Res, Fdn Inbiomed, Mesenchymal Stem Cell Lab, Paseo Mikeletegi 81, San Sebastian 20009, Spain