In vitro cytogenetic and genotoxic effects of curcumin on human peripheral blood lymphocytes.

Autores de IIS La Fe

• Natividad Sebastiá Fabregat

  Autor

• José Miguel Soriano Del Castillo

  Autor

• Joan Francesc Barquinero Estruch

  Autor

• Juan Ignacio Villaescusa Blanca

  Autor

• Miguel Jose Almonacid Bujeda

  Autor

• José Vicente Cervera Zamora

  Autor

• Esperanza Such Taboada

  Autor

• María Angeles Silla Company

  Autor

• Alegría Montoro Pastor

  Autor

Grupos

• Hematología y Hemoterapia

  

  Investigador Principal

  Javier De La Rubia Comos

Abstract

Curcumin has shown a wide range of properties such as anti-inflammatory and anti-carcinogenic properties. Many of these effects, mainly the anti-carcinogenic effect, could be linked to its anti-oxidant effects. Nevertheless, some studies suggest that this natural compound possesses both pro- and anti-oxidative effects and that curcumin could be a genotoxic agent for some cell lines. We evaluated the genetic damage induced by curcumin to human lymphocytes exposed to increasing concentrations (0-50 µg/ml) of curcumin. Biomarkers such as chromosome aberrations (CAs) and sister chromatid exchange (SCE) were analyzed. In addition to the cytogenetic analysis, the effect of curcumin in the cell proliferation kinetics (CPK) by the proliferation index (PI) was also analyzed. The results indicated that high concentrations of curcumin induced CAs, mainly acentric fragments. SCEs rate was not statistically different from the control group in any curcumin treated cell group. The PI of cells treated with 2 and 5 µg/ml of curcumin were statistically significant from the control group and finally, the MI showed a tendency to increase in all the concentrations of curcumin tested. In conclusion, it can be assumed that the higher concentrations of curcumin evaluated have a cyto and genotoxic effect, in vitro, for human peripheral lymphocytes.