Portal de investigación
The Q-LAMP Method Represents a Valid and Rapid Alternative for the Detection of the BCR-ABL1 Rearrangement in Philadelphia-Positive Leukemias.
Fecha de publicación:
Autores de IIS La Fe
Participantes ajenos a IIS La Fe
- Stella S
- Gottardi EM
- Favout V
- Barragan Gonzalez E
- Errichiello S
- Vitale SR
- Fava C
- Luciano L
- Stagno F
- Grimaldi F
- Pironi L
- Vigneri P
- Izzo B
Grupos
Abstract
Molecular detection of the BCR-ABL1 fusion transcripts is necessary for the genetic confirmation of a chronic myeloid leukemia diagnosis and for the risk classification of acute lymphoblastic leukemia. BCR-ABL1 mRNAs are usually identified using a conventional RT-PCR technique according to the BIOMED-1 method. In this study, we evaluated 122 BCR-ABL1 -positive samples with the Q-LAMP assay to establish if this technology may represent a valid alternative to the qualitative BIOMED-1 PCR technique usually employed for the detection and the discrimination of the common BCR-ABL1 transcripts (p190 and p210 isoforms). We found a 100% concordance rate between the two methods. Specifically, the p190- and p210-positive samples were amplified by Q-LAMP with a median threshold time (Tt) of 26.70 min (range: 24.45-31.80 min) and 20.26 min (range: 15.25-34.57 min), respectively. A median time of 19.63 was observed in samples displaying both (e13a2/e14a2) p210 isoforms. Moreover, the Q-LAMP assay allowed recognition of the BCR-ABL1 e13a2 and e14a2 isoforms (median Tts 18.48 for e13a2 vs. 26.08 min for e14a2; p < 0.001). Finally, 20 samples harboring rare BCR-ABL1 isoforms (e1a3, e13a3, e14a3, and e19a2) were correctly identified by the Q-LAMP assay. We conclude that the Q-LAMP assay may represent a faster and valid alternative to the qualitative BIOMED-1 RT-PCR for the diagnosis at BCR-ABL1 -positive leukemias, especially when samples are analyzed in centers with restricted resources and/or limited technical expertise.
Datos de la publicación
- ISSN/ISSNe:
- 1661-6596, 1661-6596
- Tipo:
- Article
- Páginas:
- -
- DOI:
- 10.3390/ijms20246106
- PubMed:
- 31817063
- Factor de Impacto:
- 1,317 SCImago ℠
- Cuartil:
- Q1 SCImago ℠
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES MDPI AG
Citas Recibidas en Web of Science: 7
Documentos
- No hay documentos
Filiaciones
Keywords
- BCR-ABL1, Q-LAMP, chronic myeloid leukemia, e13a2, e14a2, rare transcripts
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Cita
Stella S,Gottardi EM,Favout V,Barragan E,Errichiello S,Vitale SR,Fava C,Luciano L,Stagno F,Grimaldi F,Pironi L,SARGAS C,Vigneri P,Izzo B. The Q-LAMP Method Represents a Valid and Rapid Alternative for the Detection of the BCR-ABL1 Rearrangement in Philadelphia-Positive Leukemias. Int. J. Mol. Sci. 2019. 20. (24):6106. IF:4,556. (1).