Biomimetic microspheres for 3D mesenchymal stem cell culture and characterization

Autores de IIS La Fe

• Lourdes Cordón Gallego

  Autor

• Amparo Sempere Talens

  Autor

Participantes ajenos a IIS La Fe

• Clara-Trujillo, S
• Marin-Paya, JC
• Ferrer, GG
• Ribelles, JLG

Grupos

• Hematología y Hemoterapia

Abstract

Stem cells reside in niches, specialized microenvironments that sustain and regulate their fate. Extracellular matrix (ECM), paracrine factors or other cells are key niche regulating elements. As the conventional 2D cell culture lacks these elements, it can alter the properties of naive stem cells. In this work we designed a novel biomimetic microenvironment for cell culture, consisting of magnetic microspheres, prepared with acrylates and acrylic acid copolymers and functionalized with fibronectin or hyaluronic acid as ECM coatings. To characterize cell proliferation and adhesion, porcine mesenchymal stem cells (MSCs) were grown with the different microspheres. The results showed that the 3D environments presented similar proliferation to the 2D culture and that fibronectin allows cell adhesion, while hyaluronic acid hinders it. In the 3D environments, cells reorganize the microspheres to grow in aggregates, highlighting the advantages of microspheres as 3D environments and allowing the cells to adapt the environment to their requirements.

Keywords

• 3D environment; Extracellular matrix coatings; Microspheres; Mesenchymal stem cells; Surface modification