Portal de investigación
Whole Blood Gene Expression Reveals Specific Transcriptome Changes in Neonatal Encephalopathy
Fecha de publicación:
Autores de IIS La Fe
Participantes ajenos a IIS La Fe
- Montaldo, Paolo
- Kaforou, Myrsini
- Pollara, Gabriele
- Panadero, Joaquin
- Lally, Peter J.
- Oliveira, Vania
- Kage, Anup
- Atreja, Gaurav
- Mendoza, Josephine
- Soe, Aung
- Pattnayak, Santosh
- Shankaran, Seetha
- Herberg, Jethro
- Thayyil, Sudhin
Grupos
Abstract
Background: Variable responses to hypothermic neuroprotection are related to the clinical heterogeneity of encephalopathic babies; hence better disease stratification may facilitate the development of individualized neuroprotective therapies. Objectives: We examined if whole blood gene expression analysis can identify specific transcriptome profiles in neonatal encephalopathy. Material and Methods: We performed next-generation sequencing on whole blood RNA from 12 babies with neonatal encephalopathy and 6 time-matched healthy term babies. Genes significantly differentially expressed between encephalopathic and control babies were identified. This set of genes was then compared to the host RNA response in septic neonates and subjected to pathway analysis. Results: We identified 950 statistically significant genes discriminating perfectly between healthy controls and neonatal encephalopathy. The major pathways in neonatal encephalopathy were axonal guidance signaling (p = 0.0009), granulocyte adhesion and diapedesis (p = 0.003), IL-12 signaling and production in macrophages (p = 0.003), and hypoxia-inducible factor 1 alpha signaling (p = 0.004). There were only 137 genes in common between neonatal encephalopathy and bacterial sepsis sets. Conclusion: Babies with neonatal encephalopathy have striking differences in gene expression profiles compared with healthy control and septic babies. Gene expression profiles may be useful for disease stratification and for developing personalized neuroprotective therapies. (C) The Author(s). Published by S. Karger AG, Basel
Datos de la publicación
- ISSN/ISSNe:
- 1661-7800, 1661-7819
- Tipo:
- Article
- Páginas:
- 68-76
- DOI:
- 10.1159/000492420
- PubMed:
- 30304723
- Factor de Impacto:
- 1,178 SCImago ℠
- Cuartil:
- Q1 SCImago ℠
Neonatology KARGER
Citas Recibidas en Web of Science: 20
Documentos
- No hay documentos
Filiaciones
Keywords
- Neonatal encephalopathy; Gene expression; Brain injury; Biomarkers
Cita
Montaldo P,Kaforou M,Pollara G,HERVAS D,CALABRIA I,Panadero J,PEDROLA L,Lally PJ,Oliveira V,Kage A,Atreja G,Mendoza J,Soe A,Pattnayak S,Shankaran S,VENTO M,Herberg J,Thayyil S. Whole Blood Gene Expression Reveals Specific Transcriptome Changes in Neonatal Encephalopathy. Neonatology. 2019. 115. (1):p. 68-76. IF:2,742. (1).