Endometrial gene expression differences in women with coronavirus disease 2019

Autores de IIS La Fe

• Lucia De Miguel Gómez

  Autor

• Patricia Sebastián León

  Autor

• Mónica Romeu Villarroya

  Autor

• Nuria Pellicer De Castellvi

  Autor

• Amparo Faus Esteve

  Autor

• Antonio Pellicer Martínez

  Autor

• Patricia Díaz Gimeno

  Autor

• Irene Cervelló Alcaraz

  Autor

Grupos

• Biología Reproductiva y Bioingeniería en Reproducción Humana

• Biomarcadores, Medicina Genómica, Estadística y Análisis masivo de datos en Reproducción humana asistida

• Medicina Fetal y Reproductiva

Abstract

Objective: To study the potential effect of coronavirus disease (COVID-19) on the endometrial transcriptome of affected, symptomatic women for the detection of altered gene expression. Design: Pilot study of the endometrial transcriptomes of women manifesting COVID-19 compared with those of women without COVID-19 undergoing hysteroscopic procedures for benign gynecologic disorders using RNA sequencing. Setting: Hospital and university laboratories. Patient(s): Women with (n = 14) and without a COVID-19 (n = 10) diagnosis based on a nasopharyngeal swab analysis using quantitative reverse-transcription polymerase chain reaction. The endometrium of the patients with COVID-19 had previously been tested for severe acute respiratory syndrome coronavirus 2 infection, revealing the absence of the virus in this tissue. Intervention(s): Endometrial biopsy sample collection. Main Outcomes Measure(s): Endometrial gene expression and functional analysis of symptomatic patients with COVID-19 vs. individuals without the infection. Result(s): The systemic disease COVID-19 altered endometrial gene expression in 75% of the women, with the patients exhibiting a preponderance of 163 up-regulated (e.g., UTS2, IFI6, IFIH1, and BNIP3) and 72 down-regulated genes (e.g., CPZ, CDH3, and IRF4) (false discovery rate<0.05). A total of 161 dysregulated functions (36 up-regulated and 125 down-regulated) were typically enriched in the endometria of the patients with COVID-19, including up-regulation in pathways involved in the development of immune responses to viruses and cytokine inflammation, reflecting elicitation of a COVID-19 response pathway. Conclusion(s): Coronavirus disease 2019 affects endometrial gene expression despite the absence of severe acute respiratory syndrome coronavirus 2 RNA in endometrial tissues. © 2022 American Society for Reproductive Medicine

Keywords

• COVID-19; Endometrium; Female; Humans; Pilot Projects; RNA; Transcriptome; cytokine; interferon induced helicase C domain containing protein 1; interferon regulatory factor 4; protein BNip3; RNA; transcriptome; RNA; transcriptome; adult; Article; clinical article; controlled study; coronavirus disease 2019; down regulation; endometrium; female; gene expression; gynecologic disease; human; human tissue; hysteroscopy; nasopharyngeal swab; nonhuman; pilot study; real time reverse transcription polymerase chain reaction; RNA sequencing; upregulation; diagnosis; endometrium; genetics; pathology